Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 17;16(6):950–963. doi: 10.1093/bib/bbv019

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2015. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Variance comparison. (A) Boxplots of the variance distribution. The variance of the log2 counts of all non-spike-in miRNAs was computed across the samples, and visualized using boxplots. The data are grouped according to the normalization method. Values beyond the 1.5 interquartile range (IQR) are not shown. A clear increase in variance is observed in the data normalized by cpm or total count scaling, while a decrease is seen in UQ or TMM normalized data. (B) Mean-variance dependency. The relationship between variance and abundance level is visualized by plotting the ratio of the variance between the normalized and unnormalized data versus the average counts. Data points below the y = 0 line have decreased variance compared with the raw data. The Lowess smoother line reveals the presence of any trends in the data. A colour version of this figure is available at BIB online: http://bib.oxfordjournals.org.