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. 2015 May 14;43(16):e102. doi: 10.1093/nar/gkv475

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Clonal distribution of IgG-Fc cell pools. (A) Specific productivity (pcd) of the 20 best clones out of 50 pre-screened clones derived from stable cell pools generated with the Caggs:BAC^Rosa26, Caggs:BAC^Hprt and Caggs:plasmid vectors. (B) FACS analysis shows eGFP fluorescence of Caggs:BAC^Rosa26 and Caggs:plasmid stable cell pools expressing eGFP compared to empty plasmid control cells. (C) Correlation between specific productivity of Caggs:BAC^Rosa26 clones and their integrated vector (gene) copy numbers (gcn) relative to Actb gcn, n = 25.