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. 2015 May 14;43(16):e102. doi: 10.1093/nar/gkv475

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Fed-batch production of CN54gp140 using an optimized BAC-based vector. Fed-batch production of CN54gp140 with the Caggs:BAC^Rosa26 vector in CHO-K1 cells. (A) Product accumulation (mg/l) and (B) viable cell densities (viable cells/ml) are compared in shake flasks and in bioreactors. (C) Long-term production stability of the Caggs:BAC^Rosa26 CN54gp140 cell line without selection pressure. (D) Quality control of purified CN54gp140 in a non-reducing SDS-PAGE.