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. 2015 Jun 30;43(16):7805–7822. doi: 10.1093/nar/gkv653

Figure 5.

Figure 5.

MiR-29b directly targets Tet1/2 in mESCs. (A) No. of miR-29b binding sites at 3′ UTRs of Tet family by bioinformatics prediction software. (B and C) Tet1/2 expression was repressed by miR-29b on both RNA and protein level in mESCs by real-time qPCR and western blot analysis. (D) The structure of pmirGLO dual luciferase vector and detection of direct binding between miR-29b and Tet1/2 3′ UTRs by luciferase reporter assay. Shown here are the site locations for the Tet1/2 inserts on pmirGLO Dual-Luciferase miRNA Target Expression Vector. Decrease in luciferase activity indicates positive binding. MiR-29 mimics and the luciferases reporter were co-transfected by transient transfection using HEK293T cells. A combination of controls were employed, including mutant vector and negative control of miR-29 mimics. Each bar in the figure represents the mean ± SEM of triplicates. *P < 0.05, **P < 0.01 and ***P < 0.0001.