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. 2015 Jun 29;43(16):7838–7849. doi: 10.1093/nar/gkv667

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — DDB2 promotes histone H3K27 trimethylation in the promoter region of the NEDD4L gene. (A and B) The ChIP assay was conducted to analyze the local enrichment of histone H3K9me3 (A) and H3K27me3 (B) in specific regions of the NEDD4L promoter, as indicated in Figure 3C, in CP70 and DDB2-overexpressing CP70 cells. The relative enrichment was quantified by normalization to input first, then normalized to the P3 of CP70 cells, which is set at 1. (C and D) DDB2 was further knocked down in DDB2-overexpressing CP70-3H cells (C), and the enrichment of H3K27me3 in the P3 region of NEDD4L promoter was analyzed using ChIP (D). N = 3, Error bars represent SD. *P < 0.05; **P < 0.01 compared with mock-transfected cells.