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. 2015 Jun 29;43(16):7838–7849. doi: 10.1093/nar/gkv667

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — DDB2 recruits EZH2 to the NEDD4L promoter region. (A and B) Co-IP was carried out to show the interaction between DDB2 and EZH2 in FLAG-tagged DDB2-stably overexpressing HeLa cells (A) and FLAG-tagged DDB2-transiently overexpressing CP70 cells (B). (C) Co-IP was carried out in CP70 cells with the anti-EZH2 antibody to show the interaction between endogenous DDB2 and EZH2. (D) The ChIP assay was conducted to analyze the effect of DDB2 on the enrichment of EZH2 in the NEDD4L promoter region in CP70 and DDB2-overexpressing CP70-3H cells. The relative enrichment was quantified by normalization to input first, then normalized to P2 of CP70 cells, which is set at 1. (E) PEO1 cells were transfected with either DDB2 or Control siRNA for 48 h; the ChIP assay was conducted to analyze the enrichment of EZH2 in the P3 region of NEDD4L promoter. The relative enrichment was calculated as described above. N = 3, Error bars represent SD. *P < 0.05; **P < 0.01 compared with vector-transfected cells.