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. 2015 Jun 29;43(16):7838–7849. doi: 10.1093/nar/gkv667

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — DDB2 promotes TGF-β-induced ovarian cancer cell growth inhibition through downregulation of NEDD4L. (A) Overexpression of DDB2 sensitized CP70 cells to TGF-β-induced cell proliferation inhibition. CP70 and DDB2-stably overexpressing CP70-3H cells were treated with TGF-β for 2 days, and the BrdU incorporation assay was conducted to determine the cell proliferation. (B) Overexpression of NEDD4L in DDB2-overexpressing cells compromised TGF-β signaling in ovarian cancer cells. NEDD4L was stably overexpressed in DDB2-stably overexpressing CP70-3H cells (3H + NEDD4L) and treated with TGF-β for 4 h. Immunoblotting analysis was conducted to determine the phosphorylation of Smad2. (C and D) CP70, CP70-DDB2-3H (3H) and CP70-DDB2 cells with NEDD4L stable overexpression (3H + NEDD4L) were treated with TGF-β for 72 h (C) and 96 h (D). Cell growth was measured using the methylene blue assay. N = 5, Error bars represent SD. *P < 0.05; **P < 0.01 compared with non-TGF-β treated cells.