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. 2015 Jul 13;43(16):7878–7887. doi: 10.1093/nar/gkv707

Figure 4.

Figure 4.

SIRT4 and HDAC10 deacetylate hSSB1. (A) HEK293T cells were transfected with FLAG-hSSB1 for 36 h, treated with TSA (2 μM) or NAM (5 mM) as indicated for 12 h, then lysed and analyzed by IP and western blotting. (B) hSSB1 was cotransfected with a series of FLAG-SIRTs into HEK293T cells for 48 h, then lysed and analyzed by HA-agarose IP and western blotting. (C) hSSB1 was cotransfected with a series of FLAG-HDACs into HEK293T cells for 48 h, then lysed and analyzed by HA-agarose IP and western blotting. (D) hSSB1 WT or K94R mutation plasmids were cotransfected with SIRT4 or HDAC10 for 48 h and acetylation levels were analyzed by western blotting.