Figure 5.

Acetylation of lysine 94 stabilizes hSSB1. (A) HEK293T cells transfected with FLAG-hSSB1 WT or K94R mutation plasmids for 36 h were incubated with 20 μg/ml cycloheximide (CHX) for the indicated periods and then analyzed by western blotting. (B) Quantitation of hSSB1 protein levels during degradation based on (A). (C) HEK293T cells were transfected with the indicated plasmids for 48 h and subjected to IP using an anti-FLAG antibody followed by western blot analysis. (D) HEK293T cells transfected with the indicated plasmids for 36 h were treated with TSA (2 μM), NAM (5 mM) or C646 (10 μM) for 12 h, subjected to IP using an anti-FLAG antibody and followed by western blot analysis.