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. 2015 Jul 13;43(16):7898–7910. doi: 10.1093/nar/gkv712

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — In vitro reconstitution of Polη–dependent PCNA ubiquitination. (A) Mono-ubiquitination reactions of PCNA were reconstituted with the indicated factors. Reaction products were analysed by western blotting with an anti-PCNA antibody. KR indicates the PCNA^K164R mutant. (B) Titration of Polη and its pip mutants. Indicated mutants were subjected to the ubiquitination assays as shown in (A). (C)Relative amounts of ubiquitinated PCNA were measured from gel images of more than three independent experiments, and the average values are plotted in the graph. Error bars show SD.