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. 2015 Jul 13;43(16):7898–7910. doi: 10.1093/nar/gkv712

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — DNA polymerase assays of Polη in a reconstituted system in vitro. (A) DNA replication reactions using singly primed M13 mp18 ssDNA were reconstituted with the indicated factors. The reaction products were resolved in 10% polyacrylamide gels containing 7 M urea, and visualized using a PhosphorImager. (B) Analysis of pip mutants of Polη. Indicated mutants were subjected to replication assays shown in (A) in the presence or absence of PCNA.