Table 1. Extent of shRNA-mediated knockdown of MRE11 and ATM as determined by quantitative real time PCR (qPCR) and western blot analysis.
GFP-7F1 | GFP-6D1 | EDS-7F2 | EDS-6J8 | ||
---|---|---|---|---|---|
shATM | qPCR | 81% | 76% | 89% | 71% |
Western blot | 90%a | 70%a | 85% | 77% | |
shMRE11 | qPCR | 77% | 74% | 80% | 80% |
Western blot | 92% | 79% | 85% | 77% |
aThese are approximations due to the diffuse nature of the ATM bands.