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. 2015 Jul 22;43(16):7931–7944. doi: 10.1093/nar/gkv722

Figure 3.

Figure 3.

SETDB1, HP1 and SUV39 function downstream of the initiation of resection. (A) Following transfection of 1BR3 hTERT cells with siRNAs targeting control (CTR), SETDB1, SUV39, HP1 with or without co-depletion with CtIP or EXO1/BLM, cells were irradiated with 3 Gy and γH2AX foci enumerated at the times indicated. Asterisks denote statistically significant differences (P<0.01; t-test). Knockdown of EXO/BLM is shown in Supplementary Figure S3A. (B) Cells treated as above were enumerated for RAD51 foci at 2 h post IR. (C and D) Following siRNA mediated knockdown as in panel A, cells were exposed to 3 Gy IR and stained with RIF-1 antibodies at the indicated times. RIF1 foci were counted in (C) G1 and (D) G2 cells. (E) The ratio of RIF1/γH2AX foci in G1 versus G2 cells at 2 and 8 h post 3 Gy IR. Data are the mean ± S.E.M of three experiments.