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. 2015 Aug 3;43(16):7971–7983. doi: 10.1093/nar/gkv755

Figure 2.

Figure 2.

Plasmids carrying pWBG749-family oriT sequences. (A) Diagrams of representatives of the three most prevalent families of large staphylococcal plasmids, pMW2, pUSA300HOUMR and pIB485 (5), showing the relative positions of resistance genes, replication genes and rep gene fragments, staphylococcal-enterotoxin genes and oriT sites (see key on left, colour coding is also applicable to part B). Please see text, Figure 3 and Supplementary Figure S4 for explanation of oriT subfamily ‘OT’ designations: OT49, OT45 and OTUNa. (B) Diagrams of plasmids SAP047A and pNewbould305 (not to scale). pNewbould305 carries cadmium-resistance genes cadDX, a rep gene and an OT45-type oriT. The pNewbould305 sequence is >90% identical to a region on plasmid SAP047A, suggesting this region represents an integration of a pNewbould305-like plasmid (BLASTN comparison was made using BRIG (43)). (C) pWBG762 carries three oriT sequences and two cadDX loci as well as several rep gene fragments, suggesting multiple acquisitions of oriT-carrying cadmium-resistance RCR plasmids. The schematic illustrates the possible mechanism of the pWBG749-mediated deletion that occurred following conjugative mobilisation of pWBG762. Transfer presumably initiated at the OT45 site and terminated at the OT49 site.