Table 1. Mobilisation of plasmids carrying cloned oriT fragments from pWBG749 and pWBG747.

		Transfer frequencya
Plasmid name	oriT source	pWBG749e	pLI50 clone
pLI50	Vector-only control	5.0 × 10−6	Not detected
		1.3 × 10−5	Not detected
		2.6 × 10−5	Not detected
pLI749a	pWBG749	1.1 × 10−5	1.4 × 10−6
		1.0 × 10−5	4.9 × 10−7
		6.3 × 10−5	1.7 × 10−7
pLI749b	pWBG749	1.3 × 10−5	3.1 × 10−7
		3.3 × 10−5	2.1 × 10−7
		8.7 × 10−5	1.0 × 10−7
pLI749d	pWBG749	3.7 × 10−6	Not detected
		4.9 × 10−5	Not detected
		1.0 × 10−4	Not detected
pLI749e	pWBG749	9.7 × 10−6	1.1 × 10−6
		1.7 × 10−5	6.2 × 10−7
		5.8 × 10−5	2.6 × 10−7
pLI749f	pWBG749	2.5 × 10−5	Not detected
		2.1 × 10−5	Not detected
		1.4 × 10−4	Not detected
pLI747a	pWBG747	1.1 × 10−5	1.4 × 10−7
		1.0 × 10−5	6.0 × 10−7
		8.9 × 10−6	8.0 × 10−8
pLI747b	pWBG747	2.4 × 10−5	4.7 × 10−7
		2.2 × 10−5	5.4 × 10−7
		2.0 × 10−5	3.9 × 10−7
pLI747c	pWBG747	8.4 × 10−6	6.0 × 10−7
		2.3 × 10−5	3.7 × 10−7
		2.0 × 10−5	1.9 × 10−7
pLI747d	pWBG747	1.7 × 10−5	Not detected
		1.8 × 10−5	Not detected
		8.4 × 10−5	Not detected
pLI747e	pWBG747	1.5 × 10−5	2.4 × 10−7
		3.1 × 10−5	8.8 × 10−7
		4.5 × 10−5	1.0 × 10−6
pLI747f	pWBG747	2.1 × 10−5	Not detected
		3.7 × 10−5	Not detected
		1.9 × 10−5	Not detected

^aPer-donor frequencies of individual mating experiments.