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. 2015 Nov 19;11(11):e1005685. doi: 10.1371/journal.pgen.1005685

Fig 9. The lack of Tel1 does not restore DNA damage resistance and SSA in sae2Δ cells.

Fig 9

(A) DSB repair by SSA. The analysis was performed as described in Fig 4A. (B) Densitometric analysis of the product band signals. The experiment as in (A) was independently repeated three times and the mean values are represented with error bars denoting s.d. (n = 3). (C) Exponentially growing YEPR cell cultures of JKM139 derivative strains were transferred to YEPRG (time zero), followed by western blot analysis with anti-Rad53 antibodies of protein extracts prepared at the indicated time points. (D) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out onto YEPD plates with or without CPT, phleomycin or MMS. (E) ChIP analysis. Exponentially growing YEPR cell cultures of JKM139 derivative strains were transferred to YEPRG. Recruitment of Mre11-Myc compared to untagged Mre11 (no tag) at 0.2 kb from the HO-cut was determined by ChIP analysis and qPCR. In all diagrams, the ChIP signals were normalized for each time point to the amount of the corresponding input signal. The mean values are represented with error bars denoting s.d. (n = 3).