Fig 1. MHV68 infection induces SINE RNA expression with rapid kinetics.

(A) NIH3T3 cells were infected with MHV68 at an MOI of 5, and 24 hpi total RNA was primer extended for B1, B2, and 7SK RNAs. (B) The RNA described in (A) was used to monitor the levels of tRNA^Val, tRNA^Leu, 5S rRNA, and 7SL RNA by small RNA northern blotting. Heat shock (HS) was used as an additional control. (C) RNA isolated from subcellular compartments of NIH3T3 cells infected with MHV68 for 24 h were primer extended (B1 and B2 RNAs) or northern blotted (7SK snRNA and tRNA^Val). (C, cytoplasm; N, nucleoplasm.) (D) NIH3T3s were infected with MHV68 at an MOI 5 and total RNA was isolated at the indicated time points. The levels of B1 RNA, B2 RNA, and 7SK snRNA were monitored by primer extension. * denotes the unused radiolabed primer left in the gel. (E) Nuclei were isolated form NIH3T3s 24 hpi and used for nuclear run on analysis of 7SL RNA, EBER1, and B2 SINE. (F) MEFs were infected with MHV68 an MOI of 5 and 24 hpi total RNA was isolated and the levels of various RNA Pol III RNAs were determined by northern blotting. (G) C57BL/6 mice were intranasally infected with MHV68. 5 dpi lungs were excised and total RNA was isolated. The levels of B2 SINE RNA and 5S rRNA were monitored by northern blotting.