Skip to main content
. 2015 Aug 20;470(Pt 2):207–221. doi: 10.1042/BJ20150404

Figure 5. Effect of SB415286 and rapamycin on mTOR localization.

Figure 5

HeLa cells were cultured in EBSS containing or lacking AA or alternatively having been depleted of AAs incubated in EBSS containing a 1× AA mix (refeed) for the times indicated. In some cases, cells were incubated with 100 nM rapamycin or 50 μM SB415286 for 15 min prior to and for 10 min during the AA-refeed period as indicated. Cells were fixed, and fluorescent labelling and quantification of mTOR co-localization with LAMP2 was carried out as described in the Materials and methods section. Image quantification (means ± S.E.M.) is based on data from at least ten different fields of view. Asterisks indicate significant (P<0.05) differences between indicated bars.