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. 2015 Jul 28;6(24):20002–20025. doi: 10.18632/oncotarget.4755

Figure 4. Osteoclasts treated with supernatants from activated NKs or ZOL demonstrated modulation of cell surface receptors.

Figure 4

A. Osteoclasts were generated as described in the Materials and Methods section for 17 days. Osteoclasts were then left untreated or treated with ZOL at 500 nM or 5 μM. After 4 days of treatment, surface expression of CD54, MHC-I, MHC-II, CD44, CD14, CD11b and B7H1 were determined using flow cytometric analysis after staining with the respective PE-conjugated antibodies B. Osteoclasts were left untreated or treated with ZOL (1 μM) or supernatants harvested from pre-activated NK cells cultured with monocytes and probiotic bacteria (sAJ2) for 48 hours. Surface expressions of CD54, MHC-I, MHC-II, CD44, CD14 and CD11b were determined using flow cytometric analysis after staining with the respective PE-conjugated antibodies C. Isotype control antibodies were used as control. The numbers on the right hand corner are the percentages and the mean channel fluoresence intensities for each histogram.