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. 2015 May 8;6(24):20070–20083. doi: 10.18632/oncotarget.4039

Figure 2. MiR-101 suppresses proliferation and induces apoptosis of TNBC.

Figure 2

A. MDA-MB-435 and MDA-MB-468 cell lines were transfected with miR-101 mimics, miR-101 inhibitors or their controls. Cell viability was determined using the MTT assay at 48 h post-transfection. *p < 0.05, **p < 0.01. The data represent the mean ± SD of three independent experiments. B. MDA-MB-435 and MDA-MB-468 cells were transfected with miR-101 mimics or scramble mimics (upper panel). The effect of transfection on the levels of miR-101 expression was determined by qRT-PCR. **p < 0.01 vs. control. C. Apoptosis was evaluated by analyzing Annexin V-FITC and propidium iodine staining by FACS. D. MDA-MB-435 cell lines were transfected with miR-101 mimics, miR-101 inhibitors, or control sequences. Mitochondrial membrane potential (MMP, Δψm) was determined at 48 h post-transfection. Green indicates apoptosis. E. MDA-MB-435 cells were subcutaneously injected into nude mice. Then, the effects of intratumoral injection of 40 μL of scramble, miR-101 mimic or control, or miR-101 inhibitors in PBS on tumor volume was examined. The average tumor volumes are shown (n = 5 for both experimental groups) starting from the first injection and continuing until after the mice were killed at day 32. After 32 days, the mice were euthanized, necropsies were performed, and the tumors were weighed. All data are shown as the mean ± SD, **p < 0.01.