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. 2015 May 19;6(24):20084–20098. doi: 10.18632/oncotarget.3907

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Copyright: © 2015 Hsieh et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Human m-NAD(P)-ME (70 μM, 30 mM HEPES, pH 7.4) was titrated with EA (10 mM) at 298K. The upper panel shows the raw data curve, and the lower panel shows the fitted integrated ITC data curve. The data were fitted with the “ONESites” model of the MicroCal (Northampton, MA) version of ORIGIN 7.0.