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. 2015 May 19;6(24):20190–20203. doi: 10.18632/oncotarget.4171

Figure 3. Relationship between copy number alterations and mRNA levels.

Figure 3

A. MAPK15 mRNA levels in 48 gastric cancers with matched normal tissues were analyzed by qRT-PCR. The mRNA level in each sample was normalized to the internal control of RPLP0, and fold change of MAPK15 mRNA levels was calculated as a ratio of tumor tissue to matched normal tissue. The baseline value 0 corresponds to a fold change of 1. Blue, orange, and green colors indicate samples without MAPK15 copy number change, with copy number gain, and copy number loss, respectively. B. The fold changes of MAPK15 mRNA in cancers with or without MAPK15 copy number alteration was compared using one sample t-test (P = 0.007). C. MAPK15 copy number in 16 gastric cancer cell lines was analyzed by qPCR. D. MAPK15 mRNA levels in 16 gastric cancer cell lines were measured by qRT-PCR. The mRNA level in each sample was normalized to the internal control of GAPDH. The average mRNA level in the 16 cell lines was considered as value 1, and relative values were calculated. Error bars indicate standard deviation (n = 3). E. Correlations between MAPK15 copy numbers and its mRNA levels in 16 gastric cancer cell lines were analyzed by the Pearson's correlation coefficients (P = 0.03).