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. 2015 May 8;6(24):20231–20240. doi: 10.18632/oncotarget.4047

Figure 4. Transfection of polo-like kinase 1-specific siRNA inhibits proliferation and promotes apoptosis in Philadelphia (Ph)-positive leukemia cells.

Figure 4

A. PLK1 protein expression was measured via immunoblotting with a PLK1-specific antibody. β-actin was used as a loading control. B. PLK1 and control siRNA transfected cells were incubated with imatinib for 72 h. Viable cell numbers were counted; *P < 0.05 compared with control siRNA transfected cells. These results represent three separate experiments. C. Cells were treated with imatinib for 48 h. Percentages of apoptotic cells were determined; P < 0.05 compared with control siRNA transfected cells. Results represent three independent experiments. D. Cells were treated with imatinib for 24 h. Whole extracts were examined via immunoblotting with anti-phospho ABL, phospho-Crk-L, cleaved-PARP, and β-actin antibodies. ABL, Abelson; PARP, poly (ADP-ribose) polymerase.