Figure 1. Establishment of wild type and mutant IDH1 cell lines using U87MG cells and a lentiviral vector.

A. Schematic drawing of the lentiviral IDH1 gene construct. B. Transduction efficacy of vector containing the IDH1 gene construct, evaluated by GFP. C. FACS results showing high purity of GFP-positive cells. D. Direct sequencing analysis confirming expected sequences in U87MG-IDH1^WT and U87MG-IDH1^R132H cells. E. Western blot results showing expression of IDH1^WT and IDH1^R132H from U87MG-IDH1^WT and U87MG-IDH1^R132H cells, respectively. F. 2-HG levels in U87MG-IDH1^R132H cell extracts were approximately 18-fold the levels in U87MG-IDH1^WT or U87MG-mock cells.