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. 2015 May 10;6(24):20266–20277. doi: 10.18632/oncotarget.4060

Figure 3. In vitro evidence for an association between IDH1 mutations and 5-ALA fluorescence.

Figure 3

A. PpIX concentrations after 5-ALA treatment, expressed as relative fluorescence units (RFUs) normalized against total cell protein levels. B. Accumulation of intracellular PpIX 2 hours after 5-ALA treatment. C. Confocal laser scanning microscope images taken 2 hours after 5-ALA treatment. PpIX is visible as red fluorescence, located mainly in the cytoplasm. (λex = 405nm, λex > 560nm).