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A. RT-qPCR analysis of the HIF-1α mRNA response to LPS treatment in MC-38 cells. B. Kinetics of the HIF-1α protein response to LPS and/or hypoxia as assessed by immunoblotting of 50 μg total cell extracts. β-Actin served as loading control. C. RT-qPCR analysis of the effects of LPS treatment on the canonical HIF targets gene Glut1, Ca9 and Phd3.
