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. 2015 Apr 29;6(24):20288–20301. doi: 10.18632/oncotarget.3961

Figure 6. p65/RelA signaling in HIF-1α knock-down cells.

Figure 6

A. RT-qPCR analysis of HIF-1α and p65/RelA mRNA in MC-38 cells stably transfected with shHIF1α or shMOCK_A control constructs. B. Immunoblotting of HIF-1α and p65/RelA protein after 8 hours of hypoxic exposure of shMOCK_A or shHIF1α MC-38 cells. β-Actin served as loading control. C. Immunofluorescence microscopy of p65/RelA in shMOCK_A or shHIF-1α MC-38 cells treated with 1 μg/ml LPS for 40 minutes. D. RT-qPCR analysis of the canonical NF-κB target genes Tnfa and Il6 in shMOCK_A or shHIF-1α MC-38 cells upon treatment with LPS for 1 to 8 hours.