Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 29;6(24):20327–20344. doi: 10.18632/oncotarget.3972

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2015 Ali et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

A. An example of Huh7 hepatoma cells-derived tumor within the flank of a nude athymic mouse. Three representative excised tumors from other mice are also shown. B. Representative results showing immunohistochemical staining of the tumors for human albumin (hAlb), DCLK1, α-fetoprotein (AFP) and S100A9. C. DCLK1 overexpression correlates with enhanced S100A9 expression, activation of inflammatory pathway indicated by extensive S536 phosphorylation of NFκB, and increase in c-Myc levels in the Huh7-derived tumors. One million Huh7 cells were transplanted into the flanks of nude/SCID mice. Western blot was carried out in total lysates prepared from six Huh7-derived tumors (T1 through T6, lanes 3-8). The levels were compared with the corresponding protein expression levels in the parent Huh7 cell lysates (lane 1) and Huh7-overexpressing human RFP-DCLK1 (Huh-RD, lane 2). Protein band indicated with a star in lane 2 represents recombinant RFP-DCLK1.

A. An example of Huh7 hepatoma cells-derived tumor within the flank of a nude athymic mouse. Three representative excised tumors from other mice are also shown. B. Representative results showing immunohistochemical staining of the tumors for human albumin (hAlb), DCLK1, α-fetoprotein (AFP) and S100A9. C. DCLK1 overexpression correlates with enhanced S100A9 expression, activation of inflammatory pathway indicated by extensive S536 phosphorylation of NFκB, and increase in c-Myc levels in the Huh7-derived tumors. One million Huh7 cells were transplanted into the flanks of nude/SCID mice. Western blot was carried out in total lysates prepared from six Huh7-derived tumors (T1 through T6, lanes 3-8). The levels were compared with the corresponding protein expression levels in the parent Huh7 cell lysates (lane 1) and Huh7-overexpressing human RFP-DCLK1 (Huh-RD, lane 2). Protein band indicated with a star in lane 2 represents recombinant RFP-DCLK1.