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. 2015 May 9;6(24):20404–20418. doi: 10.18632/oncotarget.4057

Figure 4. AFAP1-AS1 knockdown didn't affect cell viability cell cycle distribution and apoptosis in vitro.

Figure 4

A. Cell viability assays using 5-8F, HK1 and HNE2 cells. Cells were grown and transfected with AFAP1-AS1 siRNA or control siRNA and then subjected to cell viability MTT assays. AFAP1-AS1 knockdown had no effect on cancer cell viability. B. Cell cycle distribution and apoptosis detection. 5-8F, HK1 and HNE2 cells were grown and transfected with AFAP1-AS1 or control siRNA for 36 h and then subjected to flow cytometry cell cycle and apoptosis assays. Relative to the scrambled control-siRNA-transfected cells, the AFAP1-AS1 knockdown had no significant effect on cell cycle distribution or apoptosis.