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. 2015 May 9;6(24):20404–20418. doi: 10.18632/oncotarget.4057

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Copyright: © 2015 Bo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Alignment of AFAP1-AS1 with the protein-coding AFAP1 gene on chromosome 4p16.1. Three exons (14, 15 and 16) of AFAP1 complemented the second exon of AFAP1-AS1. B. AFAP1-AS1 knockdown upregulated AFAP1 protein levels in 5-8F, HK1 and HNE2 cells when compared to the negative control (scrambled control-siRNA). C. AFAP1-AS1 knockdown did not affect AFAP1 mRNA levels in 5-8F, HK1 or HNE2 cells when compared to the negative control (scrambled control-siRNA).