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. 2015 May 12;6(24):20540–20554. doi: 10.18632/oncotarget.4110

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Copyright: © 2015 Shi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. and B. Effects of inhibition of BMP2 on Dragon-mediated cell proliferation: A. Dragon overexpression (oeDra) CT26.WT or CMT93 cells were transfected with control siRNA or BMP2 siRNA (siBMP2). 48 h after transfection, cells were collected for real-time PCR analysis for BMP2 mRNA expression; B. Control or Dragon overexpressing CT26.WT (left panel) or CMT93 (right panels) cells transfected with or without BMP2 siRNA were subjected to CCK-8 proliferation assay at 24, 48 and 72 h after transfection. Experiments were performed for 3 times independently. C. and D. Effects of inhibition of BMP4 on Dragon-mediated cell proliferation: C. Dragon overexpression (oeDra) CT26.WT or CMT93 cells were transfected with control siRNA or BMP4 siRNA (siBMP4). 48 h after transfection, cells were collected for real-time PCR analysis for BMP4 mRNA expression; D. Control or Dragon overexpression CT26.WT (left panel) or CMT93 (right panels) cells transfected with or without BMP4 siRNA were subjected to CCK-8 proliferation assay at 24, 48 and 72 h after transfection. Experiments were performed for 3 times independently. E. and F. Effects of inhibition of BMP7 on Dragon-mediated cell proliferation: E. Dragon overexpression (oeDra) CT26.WT or CMT93 cells were transfected with control siRNA or BMP7 siRNA (siBMP2). 48 h after transfection, cells were collected for real-time PCR analysis for BMP7 mRNA expression; F. Control or Dragon overexpression CT26.WT (left panel) or CMT93 (right panels) cells transfected with or without BMP7 siRNA were subjected to CCK-8 proliferation assay at 24, 48 and 72 h after transfection. Experiments were performed for 3 times independently. N.S., not significantly different (Dragon vs Dragon+siBMP2 or siBMP7); *P < 0.05 (Dragon vs Dragon+siBMP4); **P < 0.01.