Figure 4.

Wnt inhibition promotes scar resolution and rete ridges formation in healing skin. A, B) Gomori trichrome stained sections of skin from full-thickness excisional wound on backs of mice showing more linear (scarlike) extracellular matrix in (A) 211-treated sections (marked by yellow arrowheads), and more reticular collagen and organized extracellular matrix in (B) Pyr-treated sections (marked by red arrow). C, D) Picrosirius red-stained sections imaged with circularly polarized light showing difference in fiber thickness and alignment between 211-treated (C) and Pyr-treated (D) tissue. E–F) H&E-stained sections show increased rete ridge formation (black arrow) in Pyr-treated (F) compared with 211-treated (E) sections. A, B) Insets show low-magnification images for orientation. Scale bars, 50 µm.