Skip to main content
. 2015 Aug 26;29(12):4989–5005. doi: 10.1096/fj.15-273409

Figure 3.

Figure 3.

The tln2 and -2a genes are expressed in the head and somites, but not in the cardiovascular system. A–H) Both tln2 and -2a are expressed in the ectoderm and paraxial mesoderm at 13 somites, as well as the brain somites, from 24 to 45 hpf. Whole-mount in situ hybridization in embryos at 13 somites (A, B), at 24 (C–F) and 45 (G, H) hpf, with antisense tln2 (A, C, E, G) or antisense tln2a (B, D, F, H) probes. The anterior is to the left in all embryos. (A, B, E, F) Dorsal views; (C, D, G, H) lateral views. I) The tln1 gene, but neither tln2 nor -2a, is expressed in the embryonic and adult heart. RT-PCR revealed that tln2 and -2a were expressed in the whole embryo but not in embryonic and adult hearts; gapdh was the loading control. DNA marker, 100 bp ladder; mpf, months post fertilization.