(a) Expression of Irf1 and Gbp3 mRNA in pancreas from wild-type (WT), CAG-STAT1 and CAG-STAT1-CC mice left untreated (− IFN-β) or treated (+ IFN-β) for 1 d with IFN-β (2 × 105 U). (b) Survival of wild-type, CAG-STAT1 and CAG-STAT1-CC mice (n = 15–27 per group) 0–12 d after infection with various amounts (above plots) of EMCV. (c) Expression of RNA encoding EMCV RNA polymerase 3D (EMCV 3Dpol) in the heart, brain and pancreas of wild-type, CAG-STAT1 and CAG-STAT1-CC mice (n = 5–8 per group) 1 or 3 d after infection with EMCV (100 PFU). (d) Immunostaining of EMCV in tissue sections of pancreas from mice 0 or 4 d after inoculation with EMCV (100 PFU). (e) Hematoxylin-and-eosin staining of tissue sections of heart, brain and pancreas from mice 0 or 4 d after infection with EMCV (100 PFU). Scale bars (d,e), 40 μm. *P < 0.01, versus wild-type mice (unpaired t-test (a,c) or Kaplan-Meier analysis (b)). Data are representative of three independent experiments (mean and s.e.m. in a,c).