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. 2015 Oct 28;112(45):14096–14101. doi: 10.1073/pnas.1512865112

Fig. 5.

Fig. 5.

Subcellular localization of RCF3. (A) Nuclear localization of RCF3:eGFP and colocalization with DCL1, SE, and CPL1 tagged with mCherry in transiently transformed N. benthamiana leaves. (Scale bar: 5 μm.) (B and C) Interaction of RCF3 with CPL1 and CPL2 in yeast two-hybrid assays. AD, GAL4 activation domain fusions; BD, GAL4 DNA binding domain fusions; EV, AD, or BD empty vectors; −LT, medium without leucine and tryptophan; −LTH, without leucine, tryptophan, and histidine. Shown are 1:10 serial dilutions.