Fig. S3.

Effects of rcf3 mutation on miRNA accumulation and action in fully expanded leaves. (A–C) Expression of mature miRNAs, miRNA targets, and miRNA precursors as measured by RT-qPCR. Error bars indicate 2× SEM. (D) RNA blots for detection of mature miR160 and miR171, with U6 as loading control. Signal intensity was calculated with ImageJ and normalized to U6. Above each gel, the ratio of signal intensities of mutants to reporter control is noted. (E) Hierarchical clustering of normalized genome-wide small RNA coverage profiles within miRNA regions extending 20 bases either side of all mature miRNAs.