Fig. S7.

RCF3 Y2H interaction screen with miRNA factors. (A) Yeast transformants were plated on selective medium without leucine, tryptophan (−LT), and histidine (−LTH). All miRNA-related factors were cloned fused to the GAL4 activation domain (AD) whereas RCF3 to the GAL4 binding domain (BD). Serial dilutions of the yeast were plated to ensure even initial yeast density. (B) BiFC assay in N. benthamiana cells. Empty vector (EV) cotransformation was used as negative control. (Scale bars: 0.2 mm.)