Table S1.
Name | ID | Description |
35S:rLuc | pPM118 | Pro35S driving artificial miRNA targeting luciferase expressed from the same vector. The luciferase cDNA was mutated to escape the amiRNA silencing. |
miRNA-activity reporter | pPM085 | Pro35S driving artificial miRNA targeting luciferase expressed from the same vector |
gRCF3 | pFRK007 | Genomic RCF3 |
cRCF3 | pPM347 | RCF3 cDNA |
cRCF3 w/o stop | pPM348 | RCF3 cDNA without stop codon |
RCF3:eGFP | pPM350 | Genomic RCF3 without stop fusion to C-terminal citrine |
DCL1:mCherry | pPM115 | Pro35S driving mutated Cherry (mCherry) N-terminal-fusion to DCL1 |
SE:mCherry | pPM050 | Pro35S driving mutated Cherry (mCherry) N-terminal-fusion to SE |
CPL1:mCherry | pPL015 | Pro35S driving mutated Cherry (mCherry) N-terminal-fusion to CPL1 |
GAL4AD:RCF3 | pPM384 | GAL4 activation domain fusion to RCF3 |
GAL4BD:RCF3 | pPM385 | GAL4 binding domain fusion to RCF3 |
GAL4AD:CPL1 | pPM358 | GAL4 activation domain fusion to CPL1 |
GAL4BD:CPL1 | pPM359 | GAL4 binding domain fusion to CPL1 |
GAL4AD:DCL1 | pPM258 | GAL4 activation domain fusion to DCL1 |
GAL4AD:DCL4 | pPM261 | GAL4 activation domain fusion to DCL4 |
GAL4AD:SE3 | pPM275 | GAL4 activation domain fusion to SE3 |
GAL4AD:HYL1 | pPM273 | GAL4 activation domain fusion to HYL1 |
GAL4AD:HEN1 | pPM271 | GAL4 activation domain fusion to HEN1 |
GAL4AD:HASTY | pPM272 | GAL4 activation domain fusion to HASTY |
GAL4AD:ABH1 | pPM274 | GAL4 activation domain fusion to ABH1 |
GAL4AD:CPL2 | pPL004 | GAL4 activation domain fusion to CPL2 |
GAL4AD:AGO1 | pPM262 | GAL4 activation domain fusion to AGO1 |
GAL4AD:AGO7 | pPM268 | GAL4 activation domain fusion to AGO7 |
GAL4AD:AGO9 | pPM269 | GAL4 activation domain fusion to AGO9 |
GAL4AD:AGO10 | pPM270 | GAL4 activation domain fusion to AGO10 |
GAL4BD:CPL2 | pPL003 | GAL4 binding domain fusion to CPL2 |
Pro35S:mHYL1 | pPM444 | Pro35S driving mutated HYL1 phosphomimic Ser > Asp (last Ser > Glu) |
Pro35S:mHYL1 | pPM445 | Pro35S driving mutated HYL1 phosphomimic Ser > Ala |
cRCF3 | pPL036 | RCF3 (713–1959) fragment in pGEM |
Pro35S:CPL2:N Citridine | pPL022 | BiFC CPL2 construct |
Pro35S:CPL2:C Citridine | pPL023 | BiFC CPL2 construct |
Pro35S:CPL1:N Citridine | pPM362 | BiFC CPL1 construct |
Pro35S:RCF3:C Citridine | pPM388 | BiFC RCF3 construct |
Pro35S:RCF3:N Citridine | pPM389 | BiFC RCF3 construct |
Pro35S: AT2G29210:C Citridine | pPM375 | BiFC “Empty Vector” construct |
Pro35S: AT2G29210:N Citridine | pPM376 | BiFC “Empty Vector” construct |
ProRCF3:GUS | pPM378 | RCF3 promoter:GUS fusion |
Constructs for plant transformation are based on pGREEN and confer either Basta or kanamycin resistance in plants.