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. 2015 Oct 28;112(45):14096–14101. doi: 10.1073/pnas.1512865112

Table S1.

Plasmids

Name ID Description
35S:rLuc pPM118 Pro35S driving artificial miRNA targeting luciferase expressed from the same vector. The luciferase cDNA was mutated to escape the amiRNA silencing.
miRNA-activity reporter pPM085 Pro35S driving artificial miRNA targeting luciferase expressed from the same vector
gRCF3 pFRK007 Genomic RCF3
cRCF3 pPM347 RCF3 cDNA
cRCF3 w/o stop pPM348 RCF3 cDNA without stop codon
RCF3:eGFP pPM350 Genomic RCF3 without stop fusion to C-terminal citrine
DCL1:mCherry pPM115 Pro35S driving mutated Cherry (mCherry) N-terminal-fusion to DCL1
SE:mCherry pPM050 Pro35S driving mutated Cherry (mCherry) N-terminal-fusion to SE
CPL1:mCherry pPL015 Pro35S driving mutated Cherry (mCherry) N-terminal-fusion to CPL1
GAL4AD:RCF3 pPM384 GAL4 activation domain fusion to RCF3
GAL4BD:RCF3 pPM385 GAL4 binding domain fusion to RCF3
GAL4AD:CPL1 pPM358 GAL4 activation domain fusion to CPL1
GAL4BD:CPL1 pPM359 GAL4 binding domain fusion to CPL1
GAL4AD:DCL1 pPM258 GAL4 activation domain fusion to DCL1
GAL4AD:DCL4 pPM261 GAL4 activation domain fusion to DCL4
GAL4AD:SE3 pPM275 GAL4 activation domain fusion to SE3
GAL4AD:HYL1 pPM273 GAL4 activation domain fusion to HYL1
GAL4AD:HEN1 pPM271 GAL4 activation domain fusion to HEN1
GAL4AD:HASTY pPM272 GAL4 activation domain fusion to HASTY
GAL4AD:ABH1 pPM274 GAL4 activation domain fusion to ABH1
GAL4AD:CPL2 pPL004 GAL4 activation domain fusion to CPL2
GAL4AD:AGO1 pPM262 GAL4 activation domain fusion to AGO1
GAL4AD:AGO7 pPM268 GAL4 activation domain fusion to AGO7
GAL4AD:AGO9 pPM269 GAL4 activation domain fusion to AGO9
GAL4AD:AGO10 pPM270 GAL4 activation domain fusion to AGO10
GAL4BD:CPL2 pPL003 GAL4 binding domain fusion to CPL2
Pro35S:mHYL1 pPM444 Pro35S driving mutated HYL1 phosphomimic Ser > Asp (last Ser > Glu)
Pro35S:mHYL1 pPM445 Pro35S driving mutated HYL1 phosphomimic Ser > Ala
cRCF3 pPL036 RCF3 (713–1959) fragment in pGEM
Pro35S:CPL2:N Citridine pPL022 BiFC CPL2 construct
Pro35S:CPL2:C Citridine pPL023 BiFC CPL2 construct
Pro35S:CPL1:N Citridine pPM362 BiFC CPL1 construct
Pro35S:RCF3:C Citridine pPM388 BiFC RCF3 construct
Pro35S:RCF3:N Citridine pPM389 BiFC RCF3 construct
Pro35S: AT2G29210:C Citridine pPM375 BiFC “Empty Vector” construct
Pro35S: AT2G29210:N Citridine pPM376 BiFC “Empty Vector” construct
ProRCF3:GUS pPM378 RCF3 promoter:GUS fusion

Constructs for plant transformation are based on pGREEN and confer either Basta or kanamycin resistance in plants.