Table S1.
Kinetic parameters for the real-time measurement of lipid exchange, protein flipping, and lipid flipping
| Experimental Condition | Fast tau (±LCL/UCL) | Slow tau (±LCL/UCL) | Area of fast tau (±LCL/UCL) | Weighted tau (±LCL/UCL) |
| Addition of PE | ||||
| Lipid exchange | 8.1 ± 0.9/0.4 | 42 ± 7/3 | 0.52 ± 0.03/0.04 | 25 ± 5/0.2 |
| Protein flipping | ||||
| C6 | 34 ± 0.8 | 404 ± 10 | 0.60 ± 0.02 | 181 ± 10 |
| NT | 59 ± 4 | 577 ± 24 | 0.22 ± 0.01 | 458 ± 22 |
| P7 | 47 ± 10 | 710 ± 97 | 0.22 ± 0.03 | 564 ± 100/97 |
| Lipid flipping | ||||
| LacY (flipping) | 36 ± 2 | 186 ± 3 | 0.39 ± 0.01 | 128 ± 14 |
| LacY (no flipping) | 61 ± 4 | 459 ± 20 | 0.29 ± 0.01 | 341 ± 23 |
| CscB (no flipping) | 53 ± 2 | 508 ± 14 | 0.31 ± 0.01 | 370 ± 40 |
| Dilution of PE | ||||
| Lipid exchange | 9.7 ± 1.2/0.4 | 79 ± 7.4/0.2 | 0.66 ± 0.2 | 33 ± 3/1 |
| Protein flipping | ||||
| C6 | 26 ± 0.9 | 296 ± 12 | 0.79 ± 0.04 | 83 ± 14/13 |
| NT | 57 ± 3 | 521 ± 16 | 0.28 ± 0.01 | 391 ± 30 |
| P7 | 2,035 ± 97 | 0 | 1 | 2,035 ± 97 |
| Lipid flipping | ||||
| LacY (flipping) | 61 ± 4 | 590 ± 26 | 0.21 ± 0.01 | 477 ± 19 |
| LacY (no flipping) | 54 ± 3 | 332 ± 9 | 0.37 ± 0.01 | 230 ± 26 |
| CscB (no flipping) | 54 ± 2 | 390 ± 7 | 0.34 ± 0.01 | 276 ± 24 |
| Addition of PC | ||||
| Lipid exchange | 7 ± 0.9 | 44 ± 2 | 0.97 ± 0.03/0.01 | 7.9 ± 1.4/2.4 |
| Protein flipping | ||||
| C6 | 28 ± 0.9 | 437 ± 7 | 0.59 ± 0.01 | 197 ± 18 |
| NT | 30 ± 0.7 | 602 ± 8 | 0.49 ± 0.01 | 320 ± 39 |
| P7 | 1,180 ± 161/140 | 0 | 1 | 1,180 ± 161/140 |
| Dilution of PC | ||||
| Lipid exchange | 10.1 ± 0.5 | 66 ± 3 | 0.97 ± 0.01 | 11.9 ± 0.8 |
| Protein flipping | ||||
| C6 | 29 ± 0.7 | 363 ± 9 | 0.66 ± 0.01 | 142 ± 12/14 |
| NT | 33.3 ± 0.7 | 403 ± 9 | 0.61 ± 0.01 | 178 ± 22 |
| P7 | 59 ± 13 | 801 ± 140 | 0.19 ± 0.01 | 661 ± 120 |
Weighted Tau = Percent Areafast * Taufast + (1 − Percent Areafast) * Tauslow. LCL, lower confidence limit from exponential fits of the data; UCL, upper confidence limit from exponential fits of the data.