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. 2015 Oct 27;112(45):13862–13867. doi: 10.1073/pnas.1512959112

Fig. S4.

Fig. S4.

Model for the +5 nucleotide recognition by HigB that predicts G5 and U5 are incompatible. (A) A5 forms hydrogen bonds with the backbone of HigB, as seen in our 70S-HigB precleavage state structure. (B) Model for how G5 interacts with the HigB backbone. Note the guanosine O6 atom and the backbone carbonyl of K57 are unable to form a hydrogen bond (red X). (C) C5 forms hydrogen bonds with the backbone of HigB, as seen in our 70S-HigB precleavage state structure. (D) Model for how U5 interacts with the HigB backbone. Note the uracil O4 and N3 atoms and the backbone carbonyl of K57 are unable to form hydrogen bonds (red Xs).