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. 2015 Oct 27;112(45):13862–13867. doi: 10.1073/pnas.1512959112

Fig. S6.

Fig. S6.

Rebuild of the 70S-YoeB structure. (A) Examination of the electron density of the 70S-YoeB precleavage state structure (PDB ID code 4V8X) suggests an alternative modeling of the first two A-site nucleotides. Original mRNA model (dark gray) with electron density shown. (B) Original model (dark gray) (Left), rebuilt model (light gray) (Right), and difference electron density from refinement of 4V8X without mRNA nucleotides +4 to +8. (C) Rebuilt mRNA model and electron density map resulting from refinement. (D) mRNA paths when HigB binds to the A site (this study) and when RelE (E) binds the A site (PDB ID code 4V7J), revealing similar paths when ribosome-dependent toxins YoeB, RelE, and HigB bind the 70S.