Table S3.
mRNAs used for structural and biochemical analysis
| mRNAs, 5′–3′ | A-site codon | Assay(s) used |
| GGCAAGGAGGUAAAAAUGAAAUAGU | AAA | mRNA cleavage assay WT HigB postcleavage structure |
| GGCAAGGAGGUAAAAAUGAmAmAmUAGU | AmAmAm | 70S-HigB ΔH92 precleavage sate structure |
| GGCAAGGAGGUAAAAAUGGAAUAG | GAA | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGCAAUAGU | CAA | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGUAACAGA | UAA | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGAGAUAGU | AGA | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGACAUAGU | ACA | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGAUAUAGU | AUA | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGAAGUAGU | AAG | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGAACUAGU | AAC | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGAAUCAGA | AAU | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGCCAGAAA | CCA | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGCCCGAAA | CCC | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGAUUUAGU | AUU | mRNA cleavage assay |
| GGCAAGGAGGUAAAAAUGAmCmAmUUU | AmCmAm | HigB ΔH92 precleavage state structure |
Nucleotides followed by “m” contain a 2′-OCH3 group used to prevent mRNA cleavage during crystallization.