Fig. 3.

DC-normalization of TLR7 prevents yaa-associated splenic autoimmune traits. Sle1, Sle1Tg7, and CD11cSle1Tg7 mice were analyzed for: (A) Splenic leukocyte counts; major splenic subsets using CD4 and CD8 for T cells, B220, and CD19 for B cells and CD11b for the myeloid lineage. (B) Marginal zone (MZ) B-cell subsets were identified through CD21 and CD23 expression on B220⁺CD138⁻ cells (18). (C) T_fh were identified as CD4⁺, CXCR5⁺, and ICOS⁺. (D) Naïve and effector memory CD4⁺ T cells were gated using CD62L and CD44 expression with (E) the frequencies of CD4⁺ (Left) and CD8⁺ T cells (Right). (F) Flow cytometric analysis of splenic pDC and CD8⁺ DC subsets. (G) Myeloid CD11c⁺ populations (CD19/CD3/Gr-1⁻CD11b⁺), were subgated through MHCII and CD11c into CD11c⁺MHCII⁺ (CD11b⁺ cDCs) and CD11c⁺MHCII⁻ cells. (H) Quantification of data from G. Data shown is cumulative from three independent aging cohorts, total n = 9–10 mice per strain (A–H). Plots are representative of one mouse per strain (B, D, and G). Bars represent mean + SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; and ns, not significant (one-way ANOVA or Kruskal–Wallis test).