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. 2015 Oct 26;112(45):E6148–E6157. doi: 10.1073/pnas.1509416112

Fig. 3.

Fig. 3.

Effects of intragenic suppressors on isp-1(qm150) induction of the mitochondrial unfolded protein response, sensitivity to hyperoxia, and sensitivity to hypoxia. (A) Suppressors attenuated the hsp-6p::gfp expression (mtUPR response). Epifluorescence and DIC Nomarski images of the worms expressing hsp-6p::gfp. (B) hsp-6p::gfp fluorescence intensity of the strains were compared by ImageJ software (50). (C) Intragenic suppressors partially rescued isp-1(qm150) hyperoxia lethality. N2 at 100% O2 produced next generation. isp-1(qm150) at 21% O2 produced next generation. isp-1(qm150) died at L1 stage at 100% O2. Intragenic suppressors reached to L3/4 stage under 100% O2. Images are from day eight from synchronized L1 seeded onto NGM-OP50 at 25 °C. (D and E) COPAS sorted worms in hyperoxia and hypoxia. N2 worms were sorted at day two and other strains at day five at 25 °C. Please note that L1 in N2 are from the next generation. Error bars: SEM for highlighted experiments, *P < 0.05 **P < 0.01 ***P < 0.001.