Fig. 2.
Heterochromatin enrichment mainly occurs on the X chromosome after CDK12 knockdown. (A) HP1 spreads to the X chromosome in male flies after CDK12 depletion. Polytene chromosomes isolated from male CDK12-depleted and control larvae were stained with anti-HP1 (red), antiacetylation of histone H4 on Lys16 (anti-H4K16ac; green), and DAPI (blue). The relative fluorescence intensities of HP1 on the X chromosome based on raw data are shown. Error bars indicate SD (n = 3). **P < 0.01. (B) HP1 enrichment occurs in band regions on the X chromosome; tub-GAL80ts was combined with the SG-GAL4 to allow low levels of GAL4 activity at 25 °C. The presented chromosome is assembled from three immunostained X chromosomes. The photographic map of the X chromosome is reproduced from ref. 40, with permission from Elsevier. CON, control. (Scale bar: 10 μm.)