Fig. S3.

CDK12 depletion induces heterochromatin enrichment on the gene body of euchromatic genes with multiple exons. (A) The distribution of increased binding peaks of HP1 (black) or H3K9me2 (gray) at different regulatory regions of genes is shown. (B) The comparison of all Drosophila genes and genes with increased HP1 or H3K9me2 binding with regard to the exon numbers is shown. The data for the exon numbers were obtained from the University of California at Santa Cruz Genome Browser. (C) ChIP-seq analysis reveals the ectopic accumulation pattern of HP1 and H3K9me2 at the individual gene level in both controls (green) and CDK12-depleted (red) larvae. Heterochromatin landscape in six genes on the X chromosome is displayed. The relative positions of transposable elements (TEs; black) in the reference genes (blue) are also shown. The direction of gene transcription is indicated by the arrows.