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qPCR analysis of the expression of Ltbp-1, -3, and -4 in ascending aortas from 2-mo-old Wt, Ltbp3−/−, Fbn1mgR/mgR, and Fbn1mgR/mgR:Ltbp3−/− mice. Total RNA was isolated, and mRNA expression levels were normalized to Gapdh. Data are presented as the mean ± SD of three to five aortas per genotype. ††P < 0.01; ANOVA, P < 0.02. NS, not significant.
