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. 2015 Oct 28;112(45):E6166–E6174. doi: 10.1073/pnas.1519384112

Fig. 3.

Fig. 3.

Impaired autophagy and p62 accumulation in Atg7∆pan pancreata. (A) Autophagic flux in Atg7F/F and Atg7Δpan primary acinar cells treated or not with the ER stress-inducer tunicamycin (10 μg/mL, 4 h) in the absence (not treated, NT) or presence of the autophagy inhibitor 3-MA (10 mM), and MG132 (100 nM). Results are means ± SEM of triplicates. n = 3–4 mice per condition. *P < 0.05. (B) IB analysis of LC3B and p62 in pancreatic lysates from 12-wk-old mice. (C) p62 IHC of pancreatic sections from 12-wk-old mice of the indicated genotype. (Scale bars, 100 μm.) (D) qPCR analysis of p62 mRNA. Results are means ± SEM of triplicates. n = 3–4 mice per condition. *P < 0.05.