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. 2015 Oct 28;112(45):13916–13921. doi: 10.1073/pnas.1508514112

Fig. 3.

Fig. 3.

p27 controls MAPK activation in a CDK-independent manner. (A) Western blot analysis of pERK1/2 and pAKT expression in MEF p27KO cells that were serum starved and then stimulated with 10% serum (FBS) for the indicated times, in the presence or absence of the MEK1 inhibitor U0126 (U0) or the pan-CDK inhibitor roscovitine (Ros, Rosco), as indicated. (B) Quantitative RT-PCR (qRT-PCR) analysis of Egr-1 and Jun-B in p27KO MEFs treated as described in A. *P ≤ 0.007. (C) qRT-PCR analysis of Egr-1 and Jun-B in 3T3 fibroblasts of the indicated genotypes and in 3T3 p27KO cells stably expressing p27WT, p27CK−, or p27KR. Cells were serum-starved and then stimulated with 10% serum (FBS) for the indicated times. *P ≤ 0.04.