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. 2015 Oct 26;112(45):14066–14071. doi: 10.1073/pnas.1517371112

Fig. S5.

Fig. S5.

Anatomical defects in homozygous Sox4 or Sox11 CKO mice. (A) An E17.5 Sox4 CKO embryo (Left) displays a relatively normal brain (Upper Right) and kidneys and bladder (Lower Right). (Scale bars, 1 μm.) (B) The same organs are shown for an E17.5 Sox11 CKO embryo, which also is grossly normal. (C) A whole-mount preparation of the inner ear from a Sox4 CKO mouse shows the cochlea (C), sacculus (S), utricle (U), and ampullae (white asterisks) of the posterior (left asterisk), horizontal (center asterisk), and anterior (right asterisk) semicircular canals. (Scale bar, 500 μm.) (D) A preparation of the inner ear from a Sox11 CKO mouse reveals similar structures. (E, Upper Left) Supporting cells in the utricular macula (white outline) and cristae (white asterisks) of the anterior and horizontal semicircular canals are immunolabeled for Sox2 (green). (Upper Right) Hair cells are immunolabeled for Myo7A (magenta). The proliferating supporting cells at the edge of the utricle are labeled with EdU (Lower Left, white) and are enlarged twofold in the image at the Lower Right. (Scale bar, 100 μm.) (F) Identical labeling of the utricle and cristae in a Sox11 CKO animal reveals similar structures. (G, Upper) In the organ of Corti from an E17.5 Sox4 CKO embryo, supporting cells are immunolabeled for Sox2 (green). (Lower) The four rows of hair cells are labeled for Myo7A (magenta). The supporting cells are misaligned; some outer hair cells are absent (upper arrowhead), and ectopic inner hair cells are present (lower arrowhead). (Scale bar, 50 μm.) (H) The cochlea of an identically labeled Sox11 CKO embryo of the same age demonstrates similar defects.